向日葵未成熟胚培养中不定芽的发生

A Study on Adventitious Bud Regeneration in Immature Embryo Culture of Sunflower

研究了向日葵未成熟胚培养中不定芽的发生情况,结果表明,向日葵子叶(?)未成熟胚再生不定芽的能力很强,下胚轴较子叶容易产生不定芽;基因型和培养基是影响不定芽再生频率的重要因子.基因型不同,其不定芽的再生频率差异很大.本实验中大多数基因型都能诱导出不定芽.再生频率在40%以上.最高的达91%.自交系、杂一代和保持系的再生频率高于普通品种.培养基中高含量蔗糖及适宜的6—BA含量有利于不定芽的发生.由不定芽培育出的绿苗在温室条件下,大部分可以正常开花结实.

The study of adventitious buds regeneration in immature embryo culture of sunflower (Helianthus annuus L. ) was conducted. The experimental results indicated : (1) Im-mature embryos of sunflower at cotyledon stage had a strong ability to regenerate adventitious buds. It was easier for hypocotyl to regenerate adventitious buds than for cotyledon. (2)Genotype and medium were the critical factors ditermining the regeneration frequency of adventitious buds. Adventitious buds could be induced in most of the genotypes in the experiments. The average of the regeneration frequency was over 40%. The highest one was over 90%. The regeneration frequency of R-lines .hybrid F, and B-lines were higher than that of the common varieties. High content of sucrose. and suitable content of 6 - BA in the media were favorable for adventitious bud regeneration. Adventitious buds could be reduced by ten kinds of media used in the experiment. (3)Most of the plants developed from the adventitious buds could normally produce seeds in greenhouse.