2,4-D、KT对棉花愈伤组织的诱导和体细胞胚胎发生的影响

The Effect of 2,4-D and KT on Callus Initiation and Somatic Embryogenesis in Cotton Culture

诱导棉花下胚轴切段形成愈伤组织需要外源2,4-D,加入激动素(KT)可以增进2,4-D的效果.愈伤组织形成胚性细胞和胚性细胞团的过程需要2,4-D和KT的共同作用.KT可以促进胚性细胞团分化出胚状体,加入2,4-D则会降低KT的效果.采用2,4-D(0.05mg/L)+KT(0.1mg/L)诱导愈伤组织,60天后转移到无激素的液体培养基上振荡培养,得到了大量的胚状体.

An experiment was conduclcd lo study the effect of 2,4-D and KT on callus initiation and somatic cmbryogenesis in collon (Gossypium hirsutum L.) eullurc. The results showed that 2,4-D was essential for callus initiation, and the effect of 2,4-D was enhanced with the supplcmcnl of KT. Both 2,4-D and KT were necessary for the formation of cmbryogcnic cells and cmbryogcnic cell masses. Somatic cmbryogenesis from cmbryogcnic cell masses was promoted with KT, but the action of KT was inhibited with the supplement of 2,4-D. A lot of calli were obtained on MS medium with 2,4-D 0.05mg/ L and KT 0.1mg/ L after 60 days of culture. The calli were transfercd lo the suspension culture, then many somatic cmbryoids appeared.