通过游离小孢子培养方法获得小白菜三个变种的胚胎及植株

Embryogenesis and Plant Regeneration from Isolated Microspore Cultures of Bras sic a campestris ssp. chinensis

对普通小白菜、乌塌菜和菜■三个小白菜变种的12个基因型进行了游离小孢子培养试验.从供体母株上选取2～4mm长的花蕾,机械分离小孢子并将其置于含NAA0.5mg/L、6BA0.05mg/L和蔗糖13%的NLN82液体培养基中薄层培养,先在33℃下暗培养24h,后移至25℃下继续暗培养,2周后有9个基因型形成球形期至子叶期胚.将成熟的小孢子胚移至B_5无激素琼脂培养基上,继续发育为植株.

Isolated microsporcs from three varieties of Brassica campestris ssp. chinensis: var. cammunis, var. rosularis and var. utilis, were cultured in modified NLN82 medium supplcmented with 13% (W / V) sucrose, benzyladcninc (BA) 0.05mg/ L and naphthalcneaectic acid (NAA) 0.5mg/ L in darkness at 33℃ for one day followed by cul-luring at 25℃ . After 2 weeks of culture, microspore-derived embryos ranging from the globulor to the colylcdonary stages were obtained in 9 out of 12 genotypes. Plantlcts were regenerated after transfer of the embryos to B5 medium containing 2% sucrose without plant growth regulators.