包膜对越冬期苹果枝条内源脱落酸和赤霉素含量变化的影响

Changes of Endogenous Abscisic Acid and Gibberellic Acid in Film-Enveloped Shoots of Apple Tree During Winter

用气相色谱法和高效液相色谱法测定了越冬期间不同处理苹果枝条内源脱落酸和赤霉素的含量变化.结果表明:①越冬前期包膜和露地枝条皮层的脱落酸含量高,2月20日开始迅速下降,包膜枝条下降快,3月5日至4月5日显著低于露地枝条(P<0.05);②包膜枝条营养芽内源脱落酸1月20日达1.47×10^(-3)mg·g^(-1)高峰值,在其后15d中脱落酸含量迅速下降,3月5日至4月5日维持低而稳定的水平;③越冬期间两个处理营养芽内源赤霉素呈上升趋势,包膜枝条上升快,其含量始终高于露地枝条,分别于2月5日和3月20日出现明显高峰,包膜枝条4月5日显著高于露地枝条(P<0.01).

Changes of endogenous abscisic acid(ABA) and gibbcrellic acid(GA3) in shoots of open fields and plastic film enveloped young apple tree of Malus pumila on stock of Mains micfomalus during the overwintering period were determined by GC and HPLC respectively. The results showed that the contents of ABA in shoot bark of open fields and plastic film enveloped trees were high in the earlier stage of winter, reached their peaks (1.15 × 10-3 and 1.28 × 10-3mg · g-1) on Jan. 30 and decreased quickly after Feb. 20. In the later stage of winter the ABA content of plastic film enveloped tree was lower than that of open fields tree. The content of ABA in lateral vegetative buds of plastic film enveloped tree reached its peak (1.47 × 10-3 mg · g-1) on Jan. 20, then decreased raspidly within 15 days and remained very low (about 7.8 × 10-5 mg · g-1) from Mar. 5 to Apr. 5.The content of GA3 in vegetative buds increased during the overwintering period. GA3 content of plastic film enveloped tree increased quicker than that of open fields tree and reached its peaks (8.73 × 10-5 and 1.15× 10-4 mg · g-1) on Feb. 5 and Mar. 20. The GA3 content of plastic film enveloped tree was higher than that of open fields tree on Apr. 5. It is concluded that GA3 and ABA in the shoots play an important regulatory role during the overwintering period.