肺癌细胞株肺癌相关基因差异表达分析

Analysis of the differentially expressed lung cancer associated genes of the lung cancer cell lines

目的通过芯片技术检测肺癌细胞株基因差异表达探讨肺癌的发病机制,并尝试寻找可能用于肺癌的预防、诊断和治疗的基因。方法对四株肺癌细胞(PGCl3、PAa、H1299、NiS成瘤)作肺癌相关基因的芯片检测,分析其与对照组永生化细胞株(16HBE)之间的表达差异。从检测结果中选取了三个基因作RT-PCR鉴定。结果H1299有89个基因表达改变,PAa有59个基因表达改变,PGCL3有93个基因表达改变,NiS成瘤有78个基因表达改变。经RT-PCR鉴定的三个基因:L6A在各肿瘤细胞株中呈高表达,RXRB在各肿瘤细胞中呈低表达,vimentin除在无恶性转移株PAa中无显著差异外,在其他肿瘤细胞株中均呈高表达,结果与芯片结果一致。结论肿瘤的发生发展是多基因多阶段相互作用的结果,L6抗原可能作为肿瘤的特异性标记物,vimentin可能与肺肿瘤的转移有关,RXRB的表达水平可能与肿瘤细胞的分化相关。

Objective To study the mechanism of the lung cancer and search genes that might be used for diagnosing and treating through analyzing the genes differentially expressed in lung cancer cell lines. Methods The genes differentially expressed between the lung cancer cell lines and the controls were investigated using cDNA chip, and three genes were identified with RT-PCR. Result H1299 had 89 genes expression altered and PAa 59 altered and PGCL3 93 altered and NiS transformed 16HBE 78 altered. Three genes’ expressions validated by RT-PCR were identical to the results of cDNA chip. L6 antigen was up regulated while RXRB was down regulated. Vimentin was up regulated except in the nonmetastasis cell line PAa. Conclusions The tumor’s progress is a complicated multistep process, involving a series of genes genetic alteration, which associated to proliferation and apoptosis and metastasis. L6 antigen may be as a tumor specific marker. Vimentin is related to the metastasis of lung cancer. RXRB’s expression level is related to the tumor’s differentiation.