水分胁迫对抗旱性不同的冬小麦品种叶片蛋白质影响的比较

A Comparison of the Effect of Water Stress on Proteins in Leaves of Drought-Tolerant and Drought-Sensitive Cultivars of Winter Wheat

选用两种抗旱性不同的冬小麦——太原633(抗旱)和C609(干旱敏感),研究了水分胁迫条件下植物蛋白质代谢变化的规律及与植物抗旱性的关系.双向电泳结果表明,2.0MPa PEG-6000水分胁迫引起了两品种冬小麦叶片蛋白质变化的明显差异.抗旱品种干旱诱导蛋白及其他蛋白变化早于干旱敏感品种,初步证实了抗旱性不同的品种在水分胁迫时蛋白质代谢变化的时间顺序.干旱诱导的蛋白质的变化包括干旱诱导蛋白及蛋白质含量的变化在复水后均可迅速恢复;蛋白质转录抑制剂——放线菌素D可抑制干旱诱导蛋白的产生,说明其基因表达主要在转录水平进行调控.

Two winter wheat cultivars differing in drought resistance (Taiyuan 633, drought-tolerant; C609, drought-sensitive) were used to study the changes in protein synthesis and the relation between these changes and drought resistance of plants under the water stress. The results of two-dimensional PAGE-SDS-PAGE electrophoresis showed that water stress(-2.0MPa) caused obvious differences of protein changes in leaves of two winter wheat cultivars. The water-stress-induced protein in Tai Yuan 633 presented earlier than that in C609. The results indicated that there was a close connection between the proteinchanges in drought-tolerant and drought sensitive cultivars caused by water stress and thetime of water stress. After rewatering the protein changes could recover immediatly. The experiment of using the inhibitor showed that the emergence of the water-stress-induced proteins could be inhibited. The results implied that the gene expression of the proteins relating to adaptation to water stress was regulated mainly at transcriptional level.