豆天蛾核型多角体病毒病的研究

Studies on the Nuclear Polyhedrosis Virus Disease of Clanis bilineata tsingtauica Mell

从豆天蛾(Clanis bilineata tsingtauica Mell)的幼虫中分离到(?)株核型多角体病毒,其多角体的平面图象多为六边形和近圆形,直径0.6～2.0μm,病毒粒子杆状,两端圆滑,大小约50nm×320nm.室内和田间试验表明,该株病毒对豆天蛾幼虫有比较强的致病力.对三龄幼虫的致死中浓度(LC_(50))为1.4×10^(4.8)个多角体/ml,y=3.24+0.54x.田间防治效果可达70%左右,与苏云金杆菌制剂或其他害虫病毒制剂混用,防治效果达90%以上,好于常用化学农药氧化乐果,且残效作用较长.该病毒对家蚕、柞蚕和瓢虫无致病作用.

In August 1981, a nuclear polyhedrosis virus (NPV) was isolated from dead larvae of Clanis bilineata tsingtauica Mell in the laboratory. Under electron microscopy the polyhedra were seen as nearly rounded and hexagon in shape, verying from 0.6 to 2.0 micron in diameter. The virus particles liberated from the polyhedra in 0.05 mol / L Na2CO3 and 0.05 mol / L NaCl were rod shape with rounded ends, measured 50 × 320 nm.Bioassay conducted in the laboratory showed that the medium lethal concentration(LC50) was 1.4× 104,8polyhedra / ml, y= 3.24+0.54x. Field tests showed that the mortality of the larvae may reach 70% by spraying with the polyhedral suspension. Mixed application of the CbNPV and PaNPV, or CbNPV and Bacillus thuringiensis, was tested in the field, over 90% mortality was obtained.