系统性红斑狼疮患者体液免疫和ENA酶谱变化及意义

Changes and value of humoral immune and ENA in the patients with SLE

目的 探讨体液免疫和可提取性核抗原 (ENA)酶谱在系统性红斑狼疮 (SL E)稳定期和活动期中的变化及意义。方法 应用散射速率比浊法检测 5 2例 SL E患者 (下称实验组 )以及 2 4例正常对照者 (对照组 )的Ig G、Ig A、Ig M、C3、C4 等水平 ,应用免疫印记技术检测 ENA酶谱。结果 实验组血清 Ig G、Ig A水平明显高于对照组 ,其中活动期 (实验 B组 )血清 Ig G、Ig A、Ig M高于稳定期患者 (实验 A组 )。实验组血清 C3、C4 水平明显低于对照组 ,且实验 B组 C3水平明显低于实验 A组 ,差异有显著性 (P<0 .0 1)。 4 5 .4 % SL E患者抗 SSA、抗 SSB同时阳性 ,抗 ds DNA阳性率为 32 .7%。抗 r RNP和抗 Sm抗体在实验 A、B组的阳性率有显著差异 (P<0 .0 1,<0 .0 5 ) ,其他抗体均无明显差异。结论 SL E患者行体液免疫指标及 ENA酶谱测定可提示其疾病进展情况。

Objective To investigate the changes of humoral immune and extractable nuclear antigens(ENA) in stable and activity systemic lupus erythem atosus(SLE).Methods The level of IgG,IgA,IgM,C 3,C 4 in 23 stable and 29 activity SLE patients and 24 normal controls were determined by scatter velocity turbidimetry.Anti-extractable nuclear antigens were tested using immunobloting.Results The level of IgG,IgA in SLE were higher than the control group ,the level of IgG,IgA,IgM in activity SLE were higher than that in stable SLE and control group,the level of IgG in stable SLE were higher than control group,all of them have significant difference (P<0.01,<0.05).The level of C 3,C 4 in SLE comparing with the control group were significant decreased (P<0.05),there were significant difference between the activity SLE and stable SLE(P<0.01).Both anti-SSA and anti-SSB were positive in 45.4% patients ,anti-dsDNA had 32.7% positive.There were no difference except for anti-rRNP,anti-Sm between stable SLE and activity SLE.Conclusions The progression of SLE may be showed by testing humoral immune and anti-extractable nuclear antigen.