Z-5000原子吸收光谱法检测血铅的方法学研究

Determination of Mood lead by atomic absorption method

[目的]建立一种简便、快速、准确的血铅测定方法;检测农村儿童血铅水平。[方法]取全血0.5ml,用基体改进剂NH4H2PO4、TritonX-100和HNO3进行稀释;用Z-5000石墨炉原子吸收光谱仪,全自动进样方法进行测定。[结果]方法的测定限为1.40μg/L,相对标准偏差为1.95%-3.49%.回收率为89.99%-109.8%;已用于分析503例儿童血铅,结果满意。[结论]本法为一种理想的全血铅快速测定方法。

[Objective] To develop a simple, rapid and accurate method for determination of blood lead to detect blood lead level in children in rural area. [Methods] After the 0.5 ml blood was diluted by NH4H2PO4,TritonX-100 and HNO3,the blood level of lead was determined by atomic absorption method. [Results] The detection limit was 1.40μg/L, relative SD 1.95% - 3.49% and recovery 89.99% - 109.8%.Tie method had been successfully used in 503 children. [Conclusions] This method is good for determination of blood lead level.