青钱柳对小鼠免疫功能的影响

Effects of Cyclocarya Paliurus on Immunologic Function in Mice

目的 :研究青钱柳对小鼠免疫功能的影响。方法 :以青钱柳 1 0 0 0、50 0、2 50mg/kg经口给小鼠 ,用计数法测定腹腔巨噬细胞的吞噬百分率 ;用3H TdR掺入法测定ConA诱导脾T淋巴细胞的增殖反应 ;用免疫比浊法测定小鼠血清中免疫球蛋白的含量。结果 :青钱柳高剂量组和中剂量组小鼠的吞噬百分率较生理盐水对照组明显升高 (P <0 0 5) ,而低剂量组则无明显升高 (P >0 0 5) ;青钱柳 3个剂量组对小鼠脾淋巴细胞自发反应有促进作用 (P <0 0 5) ,且高剂量组对ConA刺激的脾淋巴细胞增殖反应较对照组明显升高 (P <0 0 5) ,而中剂量组和低剂量组较对照组则无明显升高 (P >0 0 5) ;3个剂量的青钱柳对血清中IgG、IgA、IgM含量亦无明显改变 (P >0 0 5)。结论

Objective:To explore the effects of Cyclocarya paliurus on immunologic function in mice.Methods:The mice were administrated Cyclocarya paliures orally at dose 1 000 mg/kg,500 mg/kg,250 mg/kg.The method of count,~3H-TdR incorporation and immunonphelometry were used to detect the paphagorytosis percentage of peritoneal macrophage,proliferation activity of spleen T lymphocyte stimulated Con-A;the content of immunoglobulins in serum.Results:The phagocytosis percentage at middle and high group was significant higher than contron group(P<0.05),but without obvious change at low dose group(P>05).Three dose Cyclocarya paliurus had promotional effect to the spontaneous proliferation of lymphocyte(P<0.05) and the proliferation at high dose group,stimulated con-A,was significant higher than control group(P<0.05).There were no obvious change in the content of IgG,IgA,IgM of serum at three dose groups(P>0.05).Conclusion:Cyclocarya paliurus in a suitable dose extent have effect on immunologic function.