摘要
研究甲苯二异氰酸酯(TDI)对雄性小鼠生殖细胞能量代谢的影响,探讨甲苯二异氰酸酯对雄性小鼠生殖损伤的机制.用分光光度法检测睾丸组织匀浆中琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)和ATPase的活性.TDI染毒两周后SDH、LDH及ATPase的活性均受到了抑制.在本实验染毒时间和染毒剂量范围内,TDI不仅干扰睾丸组织的有氧代谢及无氧代谢的供能过程,还干扰细胞对能量的利用.提示能量代谢障碍可能是TDI对雄性生殖细胞毒作用的机理之一.
To study the effects of toluene diisocyanate (TDI) on energy metabolism in mice's germ cells and to explore the reproductive damage in male mice caused by it. Activities of succinate dehydrogenase (SDH), lactate dehydrogenase (LDH) and ATPase in homogenate of the testis were determined with spectrophotometry. It was observed that the activities of SDH, LDH and ATPase were suppressed after exposured to TDI for two weeks. TDI not only interfered with the energy-supply process of aerobic and anaerobic glycolysis, but also the energy-utilization in testis tissue in this experiment within the length and concentrations of exposure to TDI.
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2004年第5期546-548,共3页
Journal of Harbin University of Commerce：Natural Sciences Edition
基金
国家自然科学基金资助项目(30271108).
