羟基红花黄色素A对脑缺血所致大鼠脑线粒体损伤的保护作用

Protective effect of hydroxysafflor yellow A against rat cortex mitochondrial injuries induced by cerebral ischemia

目的 研究羟基红花黄色素A对脑缺血所致大鼠脑线粒体损伤的保护作用。方法 用栓线法制作大鼠大脑中动脉缺血(MCAO)模型,测定线粒体肿胀度、膜流动性、膜磷脂含量、呼吸功能、线粒体呼吸酶、超氧化物歧化酶(SOD)、丙二醛(MDA)、Ca2+等。结果 羟基红花黄色素A(10,20 mg·kg-1)能明显抑制缺血脑线粒体膜流动性的降低,膜磷脂降解,减少脑缺血引起的线粒体肿胀,抑制NADH脱氢酶、琥珀酸脱氢酶和细胞色素c氧化酶活性的降低,改善线粒体呼吸功能;同时羟基红花黄色素A能明显降低中风大鼠脑细胞线粒体MDA含量、升高SOD活性、抑制Ca2+过多摄入。结论 羟基红花黄色素A对缺血脑细胞线粒体的损伤有明显的保护作用,该作用可能与清除氧自由基、抑制脂质过氧化、拮抗Ca2+有关。

Aim To study the effects of hydroxysafflor yellow A ( HSYA) on the mitochondrial function of cortex mitochondrial during cerebral ischemia in rats. Methods Rat focal cerebral ischemia model in rats was established by ligation of middle cerebral central artery. Cortex mitochondria were isolated and prepared for the measurement of membrane fluidity, swelling, respiratory function, activities of mitochondrial respiratory enzymes and superoxide dismutase ( SOD ) , contents of phospholipid, malondial dehyde ( MDA) and Ca 2+ to evaluate the function of mitochondria. Results Focal cerebral ischemia resulted in severe neuronal mitochondrial injuries, which could be alleviated by iv HSYA (10, 20 mg ·kg -1) , and nimodipine ( Nim, 1.0 mg ·kg-1 ) . The swelling of mitochondria was ameliorated, the decomposability of membrane phospholipid was decreased, the membrane fluidity of mitochondria was increased, HSYA also significantly inhibited the decrease in the activities of respiratory enzymes and SOD of mitochondria, and the increase in MDA and Ca2+ levels caused by cerebral ischemia in rats. Conclusion HSYA showed a protective action against the cortex mitochondrial injuries in rats induced by cerebral ischemia. The mechanisms may be derived from reducing lipid peroxides, inhibiting Ca overload, scavenging free radicals and improving the energy metabolism.