摘要
目的 研究慢传输型便秘大鼠结肠肌间神经丛内胆碱能 (AchE)神经及氮能 (NOS)神经的变化 ,探讨慢传输型便秘的发病机制。方法 建立大鼠慢传输型便秘模型 ,利用铺片技术制作结肠肌间神经丛标本 ,采用组织化学技术行结肠肌间神经丛内AchE神经及NOS神经染色。结果 与正常大鼠比较 ,模型组结肠肌间神经丛内AchE阳性神经分布较稀疏 ,神经元数量较少 ,但胞体增大 ,染色加深 (阳性细胞数、核浆比例分别为 14 34± 4 5 6vs2 7 5 6± 6 6 4 ;0 5 7± 0 2 0vs 0 76± 0 18,P <0 0 1) ;NOS阳性神经元分布较稀疏 ,数量较少 ,染色较淡 ,节间束纤维减少 (阳性细胞数、弱阳性细胞数分别为 7 10± 2 0 4vs 11 16± 2 81;2 2 1± 1 38vs 0 84±0 6 7,P <0 0 1)。结论 慢传输型便秘大鼠肠神经系统存在明显的病理改变 ,结肠传输功能障碍与肠壁内胆碱能和氮能神经的病理改变和 (或 )功能障碍有关。
s Objective To study the changes in cholinergic nerves and nitrergic nerves in colonic myenteric plexus in rats with slow transit constipation. Methods Slow transit constipation model of rat was reproduced by feeding Diphenoxylate. The changes in cholinergic nerves and nitrergic nerves in colonic myenteric plexus were studied by histochemical technology. Results In the rats with slow transit constipation, the numbers of nitriergic cells and cholinergic cells in the myenteric nerves were significantly decreased compared with control group. The volume of neuron in AchE ganglia was larger than that of control. Conclusion Dysfunction of colon transit was related with the changes in cholinergic nerves and nitrergic nerves of colonic myenteric nerve plexus in rats with slow transit constipation.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第10期854-856,共3页
Medical Journal of Chinese People's Liberation Army
关键词
便秘
慢传输型
大鼠
肌间神经丛
氮能神经
胆碱能神经
slow transit constipation
rat
myenteric nerve plexus
cholinergic nerve
nitrergic nerve