期刊文献+

慢传输型便秘大鼠结肠肌间神经丛胆碱能神经及氮能神经的组化研究 被引量:16

Study on the changes in cholinergic nerves and nitrergic nerves in colonic myenteric plexus in rats with slow transit constipation
下载PDF
导出
摘要 目的 研究慢传输型便秘大鼠结肠肌间神经丛内胆碱能 (AchE)神经及氮能 (NOS)神经的变化 ,探讨慢传输型便秘的发病机制。方法 建立大鼠慢传输型便秘模型 ,利用铺片技术制作结肠肌间神经丛标本 ,采用组织化学技术行结肠肌间神经丛内AchE神经及NOS神经染色。结果 与正常大鼠比较 ,模型组结肠肌间神经丛内AchE阳性神经分布较稀疏 ,神经元数量较少 ,但胞体增大 ,染色加深 (阳性细胞数、核浆比例分别为 14 34± 4 5 6vs2 7 5 6± 6 6 4 ;0 5 7± 0 2 0vs 0 76± 0 18,P <0 0 1) ;NOS阳性神经元分布较稀疏 ,数量较少 ,染色较淡 ,节间束纤维减少 (阳性细胞数、弱阳性细胞数分别为 7 10± 2 0 4vs 11 16± 2 81;2 2 1± 1 38vs 0 84±0 6 7,P <0 0 1)。结论 慢传输型便秘大鼠肠神经系统存在明显的病理改变 ,结肠传输功能障碍与肠壁内胆碱能和氮能神经的病理改变和 (或 )功能障碍有关。 s Objective To study the changes in cholinergic nerves and nitrergic nerves in colonic myenteric plexus in rats with slow transit constipation. Methods Slow transit constipation model of rat was reproduced by feeding Diphenoxylate. The changes in cholinergic nerves and nitrergic nerves in colonic myenteric plexus were studied by histochemical technology. Results In the rats with slow transit constipation, the numbers of nitriergic cells and cholinergic cells in the myenteric nerves were significantly decreased compared with control group. The volume of neuron in AchE ganglia was larger than that of control. Conclusion Dysfunction of colon transit was related with the changes in cholinergic nerves and nitrergic nerves of colonic myenteric nerve plexus in rats with slow transit constipation.
出处 《解放军医学杂志》 CAS CSCD 北大核心 2004年第10期854-856,共3页 Medical Journal of Chinese People's Liberation Army
关键词 便秘 慢传输型 大鼠 肌间神经丛 氮能神经 胆碱能神经 slow transit constipation rat myenteric nerve plexus cholinergic nerve nitrergic nerve
  • 相关文献

参考文献7

  • 1熊抗辉,赵经纬,王百忍.还原型尼克酰胺腺嘌呤二核苷酸脱氢酶(NADPH-d)组化方法操作步骤中各种参数的选择[J].神经解剖学杂志,1996,12(1):79-82. 被引量:34
  • 2[3]Voderholzer WA, Wiebecke B, Gerum M et al . Dysplasia of the submucous nerve plexus in slow-transit constipation of adults. Eur J Gastroenterol Hepatol, 2000 ,12(7):755
  • 3[4]Wedel T, Roblick UJ, Ott V et al . Oligoneuronal hypoganglionosis in patients with idiopathic slow-transit constipation. Dis Colon Rectum, 2002 ,45(1):54
  • 4[5]Schemann M, Schaaf C, Mader M. Neurochemical coding of enteric neurons in the guinea pig stomach. J Comp Neurol,1995,353:161
  • 5[6]Tomita R, Tanjoh K, Fujisaki S et al . Regulation of the enteric nervous system in the colon of patients with slow transit constipation. Hepatogastroenterology,2002,49(48):1540
  • 6[7]Kimura H, Ito S, Ohta T. Vasoactive intestinal peptide released by acetylcholine in the dog ileum. J Auton.Nerv Syst,1994,48(7):167
  • 7[8]Tomita R, Fujisaki S, Ikeda T et al . Role of nitric oxide in the colon of patients with slow-transit constipation.Dis Colon Rectum,2002,45(5): 593

共引文献33

同被引文献166

引证文献16

二级引证文献185

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部