地塞米松对尾悬吊大鼠肺组织细胞凋亡的影响

Effects of dexamethasone on cell apoptosis in pulmonary tissues in rats after tail-suspension simulating weightlessness

目的 探讨地塞米松对尾悬吊大鼠肺组织细胞凋亡的影响。方法 采用尾悬吊大鼠模拟失重模型 ,将健康雄性SD大鼠分为尾悬吊组 (尾悬吊 7天模拟失重 )、地塞米松干预组 (尾悬吊第 3天开始腹腔注射地塞米松 5mg/kg,共 5天 )和正常对照组 ,每组 10只 ,用TUNEL技术检测肺组织细胞凋亡情况。结果 尾悬吊组凋亡指数显著高于对照组 ,差异有统计学意义 (P <0 0 5 ) ,地塞米松干预后细胞凋亡水平明显低于尾悬吊组 ,差异有统计学意义 (P <0 0 5 )。结论 尾悬吊模拟失重的大鼠肺组织细胞凋亡指数增高 ,而地塞米松可抑制模拟失重大鼠肺组织细胞的凋亡。

Objective To investigate the effects of dexamethasone on prevention of apoptosis in pulmonary tissues of rats after simulated weightlessness. Methods Tail suspension was used to simulate weightlessness. Thirty Sprague-Dawley male rats were divided randomly into 3 groups: control group, tail suspension for 7 days group, and dexamethasone treatment after suspension group. TUNEL technique was used to detect cell apoptosis in pulmonary tissues. Results The rate of apoptotic cells in the lung of rats suspended for 7 day was higher than that of control group(P<0.05). The index of pulmonary cell apoptosis in dexamethasone treatment group was lower than that in rats suspended for 7 day group(P<0.05). Conclusions Cell apoptosis index in pulmonary tissues was increased in rats after being suspended for 7 day. Dexamethasone could suppress cell apoptosis in pulmonary tissues of rats after simulated weightlessness.