人结直肠癌组织中B7和ICOS分子mRNA的表达

B7 and ICOS mRNA expression in human colorectal carcinoma

目的 :通过原位检测B7 1,B7H1,B7H2和ICOS等分子mRNA在结直肠癌细胞及肿瘤浸润淋巴细胞 (TIL)中的表达 ,以期探讨肿瘤微环境中Th2型细胞因子表达上调的可能机制 .方法 :采用原位杂交技术 ,用地高辛标记的寡核苷酸探针检测了 2 2例人结直肠癌组织B7 1,B7H1,B7H2和ICOS分子mRNA表达状况 ,并结合临床特点加以综合分析 .结果 :肿瘤组织和肿瘤浸润淋巴细胞除表达B7 1外 ,均可见B7H1,B7H2和ICOSmRNA表达 ,但肿瘤细胞的表达水平显著高于TIL的表达水平 ,具有显著性差异 (P <0 .0 5 ) ;B7家族分子在癌细胞和TIL中的表达均与患者性别、年龄、肿瘤分化程度、有无区域淋巴结转移无关 ,在TIL中B7H1表达与肿瘤浸润深度有关 (P <0 .0 5 ) ;在癌细胞中则与之无关 (P =0 .15 5 ) ;在癌细胞和TIL中B7H1和ICOS分子表达与远处转移之间具有显著性差异 (P均 <0 .0 1) .结论 :肿瘤细胞和TIL表达B7H1,B7H2和ICOS分子可能与肿瘤微环境中Th2型细胞因子占优势有关 .

AIM: To observe the tumor associated B7 family molecules mRNA expression on human colorectal cancerous tissue by in situ hybridization. METHODS: The mRNA expression patterns of cancer associated B7-1, B7H1, B7H2 and ICOS mRNA expression in 22 specimens of human colorectal cancer were monitored by the technique of in situ hybridization (ISH) with the Diogenes-labeled Oligonucleotide-probes. RESULTS: B7-1, B7H1, B7H2 and ICOS mRNA were detected in both cancer cells and tumor infiltrating lymphocytes (TILs). The mRNA expression level of these molecules in tumor cells was higher than that in TILs (0.76±0.54-1.62±0.82 vs 0.38±0.19-0.65±0.33, P< 0.01). There was no correlation between the expression level of tested B7 family molecules and the sex, age, differentiation status of cancer and regional lymph nodes metastasis of the patients. CONCLUSION: Th2 cytokine predominant in tumor microenvironment may be related to the expression of B7H1 and B7H2 co-signal molecules on tumor cells and TILs.