摘要
为了解白的消化生理过程 ,利用ABC免疫组织化学方法 ,对白胃肠道 5种内分泌细胞 :5 -羟色胺 (5 HT)、脑啡肽、P物质、胃动素和抑胃素的分布进行了研究。结果表明 :5 HT细胞主要分布于十二指肠及其以下部位 ,尤其是盲肠和直肠 ;而腺胃、肌胃和幽门中均未发现该类细胞。脑啡肽和P物质细胞数量明显较 5 HT少 ,它们稀疏分布于十二指肠及其以下部位 ,无显著的分布积聚区。抑胃素和胃动素在整个胃肠道中均无分布。分析表明白胃肠道 5种内分泌细胞的分布与其他鸟类相似 ,但与其他脊椎动物明显不同。
For further understanding the digestive physiology of white Ibis,by employment of ABC (streptavidin-peroxidase) immunohistochemical method we studied the distribution of endocrine cells containing 5-hydroxytryptamine (5-HT),met-enkephalin (ENK),substance P (SP),motin and gastric inhibiting polypeptide (GIP) in the gastrointestinal tract of white Ibis (Threskiornis melanocephalus).The results were as follows:5-HT immunoreactive cells were located throughout the gastrointestinal tract with the highest density in the cecum.However,these immunoreactive cells were nearly absent from the stomach.The cells containing ENK and SP were located in the intestine,but they were far sparser than 5-HT cells.Either ENK or SP cells were not congregated throughout the gastrointestinal tract.The cells immunoreactive to motin and GIP were not observed in the gastrointestinal tract of white Ibis.The analysis suggests that the distribution above endocrine cells in the gastrointestinal tract of white Ibis resembles in other bird species,but differs in other vertebrates.
