大鼠心肌缺血再灌注损伤实验模型研究

Rat models of myocardial ischemia reperfusion injury

目的:改进心肌缺血再灌注模型制备方法,提高造模成功率,以便使其能反复地多次进行心肌缺血再灌注实验和确保心肌缺血预处理实验顺利进行。方法:采用成年SD大鼠制作心肌缺血再灌注损伤模型,剪断肋骨同时结扎肋间动脉,不使用扩胸器,直接用结扎线将肋骨残端用力拉向两侧,充分暴露心脏;结扎冠状动脉操作在胸腔内进行。结果:用改进的方法造模成功率100%;用传统的方法造模的成功率为80%。应用此法造模进行反复多次缺血再灌实验的成功率为90%;而用传统的方法造模进行反复多次缺血再灌实验的成功率仅为10%。结论:改进后的方法简单易行,操作方便,成功率高。此法造模是目前完成心肌缺血再灌注实验比较好的模型,又是保证大鼠心肌缺血预处理实验成功的方法。

AIM:To improve the method to estabilish the model of myocardial ischemia reperfusion, and improve the successful rate,so as to repeatedly use the models in experiments and ensure that the experiment can be performed successfully. METHODS:Adult SD rats were used to make the models of myocardial ischemia reperfusion injury.The ribs of rats were cut off after arterial intercostals were ligated.The thorax dilater was not used, and instead, the stump of ribs were extracted to the opposite sides with wire ligature to expose the heart.The ligation of coronary artery was performed in the pleural(thoracic) cavity. RESULTS:The successful rate of model improvement method was 100%, and that of traditional method was 80%.The successful rate using this model establishment method in repeated ischemia reperfusion experiments was 90%; while that using traditional method was 10%. CONCLUSION:The improved method is simple and convenient,with high successful rate and the model is good for myocardial ischemia reperfusion injury experiment,and it is a successful method to succeed myocardial ischemia preconditioning experiment in rats.