黄芪对高葡萄糖、游离脂肪酸培养的人血管内皮细胞一氧化氮含量的影响

Effect of astragalus membranaceus on nitric oxide level of human vascular endothelial cells cultured with high glucose and free fatty acids

目的:探讨高葡萄糖和非酯化脂肪酸(freefattyacids,FFA)对人血管内皮细胞(vascularendothelialcell,VEC)产生的一氧化氮水平的影响和黄芪的干预作用,以及黄芪对糖尿病肾病治疗作用的可能机制。方法:以体外培养的人VEC为对象,观察以5.5mmol/L葡萄糖(对照组),10,20和30mmol/L葡萄糖,0.125,0.250和0.500mmol/L非酯化脂肪酸、高浓度葡萄糖+高浓度油酸和/或棕榈酸,2,20和200g/L黄芪,低、中、高质量浓度黄芪+高浓度葡萄糖,高浓度油酸和/或棕榈酸+低、中、高质量浓度黄芪,高浓度葡萄糖+高浓度油酸和/或棕榈酸+低、中、高质量浓度黄芪培养液培养后,VEC上清液中一氧化氮的含量。结果:VEC经含30mmol/L葡萄糖及30mmol/L葡萄糖+0.500mmol/L棕榈酸培养后一氧化氮水平明显升高,与对照组比较,差异有显著性意义(P<0.01)。经不同浓度黄芪处理后,一氧化氮升高组人VEC一氧化氮水平均有下降,以高质量浓度黄芪(200g/L)试验组最明显。相反,VEC在0.500mmol/L油酸、0.500mmol/L棕榈酸+0.500mmol/L油酸和30mmol/L葡萄糖+0.500mmol/L油酸+0.500mmol/L棕榈酸条件下培养后,一氧化氮水平明显下降,与对照组比较,差异有显著性意义(P<0.01)。经不同浓度黄芪处理后,一氧化氮下降组人VEC一氧化氮水平有不同程度升高,但与对照组相比,差异仍?

AIM:To investigate the effect of high glucose and free fatty acids(FFA) on nitric oxide(NO) produced by human vascular endothelial cells(VEC) and the intervention of astragalus membranaceus(AM),and study the possible mechanism of AM in treating diabetic nephropathy. METHODS:The human VEC cultured in vitro were used for study.NO contents in the supernate of VEC were measured after cultured in the following culture media:5.5 mmol/L glucose(control group),low,middle and high concentration glucose(10, 20 and 30 mmol/L),low, middle and high concentration oleic acid(OA) and/or palmitic acid(PA) (0.125,0.250 and 0.500 mmol/L),high concentration glucose+high concentration OA and/or PA,low,middle and high concentration A (2,20 and 200 g/L),low,middle and high concentration AM+high concentration glucose, high concentration OA and/or PA+low,middle and high concentration AM,high concentration glucose+high concentration OA and/or PA+ow,middle and high concentration AM. RESULTS: The NO levels in the VEC cultured in 30 mmol/L glucose and 30 mmol/L glucose+0.50 mmol/L palmitic acid were significantly increased as compared with that in the control group (P< 0.01).After treated with AM,the NO levels in the increasing groups were all decreased, especially in the high concentration(200 g/L) AM group.In contrast,the NO level in the supernate of 0.500 mmol/L OA group,0.500 mmol/L PA+0.500 mmol/L OA group and 30 mmol/L glucose+0.500 mmol/L OA+0.500 mmol/L PA group were significantly decreased as compared with that in the control group (P< 0.01).After treated with AM,the NO levels in the decreasing groups were increased to different degree,but still significantly different from that in the control group. CONCLUSION:AM can up regulate the abnormal changes of NO levels caused by high glucose and FFA to certain degree, and has certain effect in ameliorating the injury of VEC,which may be one of the essential mechanisms of AM in treating the pathological changes of early and late diabetic nephropathy.