皂甙对乙醇性肝损伤的保护作用

Effect of saponin in protecting against alcoholic liver injury

目的:探讨皂甙对乙醇引起的肝损伤模型大鼠肝脏的保护作用效果。方法:采用乙醇复制大鼠肝损伤模型,测定肝组织的丙二醛、三酰甘油及还原型谷胱甘肽含量;并观察肝脏的病理组织学改变。结果:受试样品高、中、低剂量组大鼠肝组织的丙二醛含量均值分别为123.8,112.5,117.6nmol/g(肝组织),均低于肝损模型组的140.0nmol/g;三酰甘油含量均值分别为2.43,2.52,2.50mmol/100g(肝组织),均低于肝损模型组的4.10mmol/100g;而还原型谷胱甘肽含量均值分别为11.39,11.53,10.99μmol/g(肝组织),均高于肝损模型组的9.40μmol/g;各剂量组大鼠肝脏病理改变评分均值分别为49.3,45.8,40.0分,均低于肝损模型组的134.1分。结论:皂甙对乙醇性引起的大鼠肝损伤具有保护作用。

AIM:To explore the protective effect of saponin on rat model of liver injury caused by alcohol. METHODS:Rat models of alcoholic liver injury were established.The contents of malondialdehyde(MDA),triacylglycerol(TG) and reduced glutathione(GSH) in liver tissue were measured,and the pathological and histological changes of liver were observed. RESULTS:The MDA contents in liver tissue in the high,middle,and low dosage groups were 123.8,112.5 and 117.6 nmol/g respectively,significantly lower than that in the model control group(140.0 nmol/g).The TG contents in liver tissue in the high, middle,and low dosage groups were 2.43,2.52 and 2.50 mmol/100 g respectively,significantly lower than that in the model control group(4.10 mmol/100 g),while the GSH contents in liver tissue in the high, middle,and low dosage groups(11.39,11.53 and 10.99 μmol/g) were significantly higher than that in the model control group(9.40 μmol/g).The average value of pathologcial changes in rat liver in the high, middle,and low dosage groups were 49.3,45.8 and 40.0 respectively,significantly lower than that in the model control group(134.1). CONCLUSION:Saponin has a significant protective effect on liver injury caused by alcohol.