番茄红素对慢性苯染毒小鼠抗氧化酶的影响(英文)

Impact of lycopene on antioxidative enzymes in mice with chronic benzolism

背景:苯中毒主要对造血系统和神经系统造成损伤,导致DNA损伤,DNA加合物形成等。番茄红素是一种重要的类胡罗卜素,具有多种生物学作用,如抗氧化、抗肿瘤、诱导细胞间隙连接通讯等。目的:探讨番茄红素对慢性苯染毒所造成的氧化损伤的拮抗作用。设计:随机对照的实验研究。地点和材料:哈尔滨医科大学公共卫生学院动物室。选用纯种雄性健康昆明鼠30只,由哈尔滨医科大学附属第二医院动物中心提供。干预措施:分为对照组、苯染毒组和番茄红素组,每组10只。对照组不染毒,苯染毒组和番茄红素组静式吸入苯蒸气,番茄红素组给予番茄红素,60d后处死,取肝、脾、脑组织。主要观察指标:测定肝、脾和脑中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛含量。结果:苯染毒能够明显,降低组织匀浆中SOD活性犤肝、脾、脑分别为(12.57±1.94),(5.57±0.81),(8.28±2.25)μkat/g〗,GSH-Px活力犤肝、脾、脑分别为(0.38±0.05),(0.52±0.05),(0.10±0.01)mkat/g犦,升高丙二醛含量犤肝、脾、脑分别为(2.85±0.38),(3.58±0.53),(3.10±0.89)μmol/g犦。给予番茄红素后可使小鼠组织匀浆的SOD活性(μkat/g)升高(肝、脾、脑分别为16.08±2.55,6.43±1.28,9.03±1.52),GSH-Px活力(mkat/g)上升(肝、脾、脑分别为0.53±0.

BACKGROUND:Benzolism(benzene poisoning) mainly hurts hemopoietic system and ne rvous system,which would induce DNA injury,and DNA adducts formation,etc.Lycopen e is an important carotenoid,which has many biological effects, such as, antioxi dation,anti carcinoma and induction linkage and communication of intercellular space,etc. OBJECTIVE:To discuss the antagonism of Lycopene in antioxidative system injury caused by chronic benzolism. DESIGN:A randomised controlled study SETTING and MATERIALS:The study was conducted in the animal laboratory of the Faculty of Public Health of Harbin Medical University.30 pure bred male healthy Kunming mice were obtained from the Experimental Animal Center of Second Hospita l Affiliated to Harbin Medical University. INTERVENTIONS:Animals were randomly allocated into normal control group, benzo lism group and Lycopene group with 10 each.Animals in control group were not poi soned,but animals in benzolism group and Lycopene group statically inhaled benze ne vapour. Lycopene was administrated only in Lycopene group but not benzolism g roup.Animals were executed after 60 days to remove liver,spleen and brain tissue s for the preparation of homogenates. MAIN OUTCOME MEASURES: The activities of Superoxide dismutase(SOD) and glutath iione peroxidase(GSH Px) as well as malondialdehyde (MDA) content in liver homo genate,spleen homogenate and brain homogenate were assayed. RESULTS:Benzolism would significantly affect the activities of antioxidative e nzymes by decreasing SOD activities[(12.57±1.94),(5.57 ±0.81),(8.28±2.25) uka t/g]and GSH Px activities[(0.38±0.05),(0.52±0.05),(0.10±0.01) mkat/g]and ele vating MDA contents[(2.85±0.38),(3.58±0.53),(3.10±0.89) μmol/g ]in the homo genates of liver,spleen and brain respectively. After the administration of Lyco pene,the activities of SOD(μkat/g) increased[(16.08±2.55),(6.43±1.28), (9.03 ±1.52)],the activities of GSH Px(mkat/g)[(0.53±0.08),(0.62±0.07),(0.12±0.01 )] elevated,and MDA contents (μmol/g)[(2.26±0.31),(2.98±0.37),(2.79±0.37)] d ecreased in the homogenates of liver,spleen and brain respectively, which all ha d significant differences compared with that of benzolism group(q =4.00-8.04,P< 0.05). CONCLUSION:Lycopene could relieve the lipid peroxidation(LPO) induced by benzo lism to improve the ability of antioxidation in mice by enhancing the activities of antioxidative enzymes for the antagonism of the oxidative injury caused by b enzolism.