乙型肝炎病毒基因型与血清HBV DNA水平的关系

The relationship between hepatitis B virus Genotypes and serum level of HBV DNA in chronic hepatitis B

目的 探讨乙型肝炎病毒 (HBV)基因型与血清HBVDNA水平的关系。方法 采用PCR、核酸杂交和酶联显色技术以及荧光定量PCR技术 ,分别对 12 3例慢性乙型肝炎 (CHB)患者进行HBVDNA基因分型和血清HBVDNA水平的测定。结果 泉州地区CHB患者的HBV基因型以B型为主 ,其次为C型 ,部分以D型和混合型存在 ,分别占 74 80 %、17 0 7%、3 2 5 %和 5 69% ,无A、E、F型。C基因型的HBVDNA水平 (log值 )为 7 0 5± 1 3 5 ,显著高于B基因型的 6 3 2± 1 0 6,P <0 0 1;而C基因型的HBeAg含量为5 3 62± 2 5 3 2 ,亦显著高于B基因型的 40 1l± 15 17,P <0 0 1。C基因型患者的TBIL、ALT、AST均显著高于B基因型 ,而白蛋白水平则显著低于B基因型 ,P <0 0 1。结论 泉州地区CHB患者以B基因型为主 ,部分以C型、D型和混合型存在。C基因型的血清HBVDNA水平显著高于B型 。

Objective To investigate the relationship between hapatitis B virus genotypes and serum level of HBV DNA.Methods The genotype of HBV and serum level of HBV DNA were detected by PCR,ELISA and quantitive fluorescent PCR in 123 patients of chronic hepatitis B(CHB) in Quanzhou area.Results Genotype B,C,D and mixed genotypes(B and D or C and D) were detected (74.80%,17.07%,3.25% and 5.69% respectively).No genotypes A,E and F were detected.The level of HBV DNA (7.15±1.35) in genotype C was much higher than that of genotype B(6.32±1.06),P<0.01.And the level of HBeAg in genotype C (53.62±25.32) was much higher than that of genotype B (40.11±15±17),P<0.01.The level of TBIL,ALT and AST of genotype B were significantly higher than that of genotype B,but the level of ALB in genotype C was significantly lower than that of genotype B.Conclusion Genotype B is the majority genotype in Quanzhou area.The serum level of HBV DNA in genotype C is significantly higher than that of genotype B.And genotype C may induce more severe the liver inflammation than genotype B may do.