胆囊结石病肝气郁结证与CCKAR和VIPR基因表达的相关性研究

Relationship between Liver-Qi-Stagnation Syndrome of Gall-Stone Disease and Gene Expression for CCKA Receptor and VIPR Receptor

目的 :探讨血管活性肠肽受体基因表达在胆囊结石病肝气郁结证形成中的作用。 方法 :术中取出胆囊结石病人胆囊体相同部位肌层组织 ,无菌生理盐水反复冲洗后 ,RT -PCR法检测胆囊组织中VIPRmRNA的表达。 结果 :与非肝气郁结证组相比 ,肝气郁结证组病人胆囊组织中VIP受体基因的表达显著增高 (P <0 0 0 1)。 结论

Objective To investigate the effect of the gene expression for vasoactive intestinal peptide receptor(VIPR) in the formation of liver qi stagnation syndrome of gall stone disease Methods Gallbladder tissue at the gallbladder calculi was obtained by operation and washed repeatedly with steril saline.The expression of VIPR mRNA in gallbladder tissue was subjected to RT PCR assay.Results In comparsion with the none liver qi stagnation syndrome,the gene expression of VIPR of gallbladder tissue increased obviously in patients with liver qi stagnation( P <0 001). Conclusion The formation of liver qi stagnation syndrome of gall stone disease is considered to be related to the high level of VIPR mRNA expression in gallbladder tissue.