比色法测定兔血浆中甘露醇浓度

Determination of the Concentration of Mannitol in Rabbit Plasma by Colorimetry

目的 :建立血浆中甘露醇浓度的测定方法。方法 :用三氯醋酸沉淀含甘露醇的血浆样品蛋白 ,血浆上清液中加入高碘酸钠后用Nash试剂显色 ,在 40 5nm波长处测定吸收度。结果 :甘露醇在 0 .188～ 6.0 0mg·mL 1 范围内有良好的线性关系 ,r =0 .9997(n =6) ,平均回收率为 98.3 0 %,RSD为 1.89%(n =3 )。结论 :该方法快速、简便 ,适用于甘露醇血药浓度测定及临床药动学研究。

Objective:To establish a method for determination of mannitol in rabbit plasma. Methods:The plasma samples was deproteinized with acetate trichloride, mannitol contained in supernatant was oxidated with sodium periodate deproteinized and further reacted with Nash reagent to form the colour product which can be detected at a wavelength of 405 nm. Results:The linear range of calibration curve was 0.188-6.00 mg·mL -1, r=0.999 7(n=6). Corresponding to the drug plasma concentration. The average rate of recovery was 98.30%. RSD was 1.89%(n=3). Conclusion:The method is rapid, simple and can be used for determination of plasma concentration of mannitol and its clinical pharmacokinetic study.