甲醛对人红细胞的影响

The Effect of Formaldehyde on Human Erythrocyte

生产、科研,生活中,有大量人群长期接触甲醛.甲醛可引起肝脏病变,致癌、致突变,已日益为人们所关注.本文采用人红细胞为材料,研究甲醛的毒害作用.人红细胞悬液,经0.1～1.0×10^(-6)mol/l甲醛处理后,通过生化分析,实验结果表明,在本实试条件下甲醛增强红细胞溶血作用,抑制乙酰胆碱酯酶活力和引起可滴定巯基含量降低.同时使磷酸甘油醛脱氢酶、乳酸脱氢酶活力提高和促进细胞内Ca^(2+)的泄漏.

In ordes to study the effect of formaldehyde on normal cell, human erythrocyte is used to investigate formaldehyde toxicity. Human erythrocyte were exposed by variaus formaldehyde concentrations: 0.1, 0.2, 0.3, 0.5, 0.75, 1.0×10^(-6)m, respectively.The cell suspensions were then incubated at 37℃ for 60 min. Experimental results show that formaldehyde causes decrease in the activity of erythrocyte ethylcholinesterase and in the number of titrable sulfhydryl groups and an increase in the activity of glyceraldehydephosphate dehydrogenase and lactate dehydogenase.The inward Ca^(2+) leak is also increased.