卡铂血药浓度高效液相色谱分析

Study on Blood Concentration of Carboplatin by HPLC Analytic Method

目的 建立一种快速、准确的卡铂血药浓度高效液相色谱分析方法 ,为药代动力学研究和药效学评价提供方法学基础。方法 色谱条件 :Spherigel C18色谱柱 ,流动相 :含 0 1%乙腈的 0 0 2mol/LKH2 PO4缓冲液 ,流速 :1mL/min ,UV :2 30nm。结果 线性范围 1mg/L～ 2 0mg/L ,相关系数r =0 990 2 ,平均回收率 97% ,最低检测浓度 0 5mg/L。结论 抗癌药卡铂的药动学存在较大个体差异 ,利用药理学原则制定卡铂适用的给药方案并通过测定血药浓度来估算卡铂血浆浓度 时间曲线下面积 (AUC)进而指导合理用药 ,具有显著的临床意义 ;本文介绍的卡铂血药浓度高效液相色谱检测方法 ,具有样品处理简单、稳定性高等特点 。

Objective To establish a rapid and precise HPLC determination method of blood concentration of carboplatin.Methods The concentration of carboplatin was assayed on a Spherigel-C 18 column with a mobile phase consisting of 0.1% acetonitrile: 0.02mol/L KH 2PO 4 at a flow rate of 1ml/min and detected at UV 230nm.Results The calibration curve was linear over the range of 1mg/L～20mg/L(r= 0.9902).The average recovery of carboplatin was 97%.The limit of quantification was 0 5mg/L.Conclusion There is relatively large differentiation in the pharmacokinetics of carboplatin.Using pharmacological principle,rational scheme of administration of carboplatin was drawn up,through the determination of blood concentration AUC of carboplatin was estimated to guide rational administration.This technique possessed significant clinical value.HPLC determination method of blood concentration of carboplatin possessed the features of simple sample treatment and high stability.Clinical application demonstrated that this method had a better practicality and can be popularized easily.