酶免疫法检测结核分枝杆菌糖脂抗体监测结核患者的体液免疫

Detection of anti-tuberculous glycolipid antibody using an enzyme-immunoassay for monitoring humoral immunity against TB

目的 探讨用酶免疫实验检测抗结核分枝杆菌糖脂抗原 (TBGL)抗体的方法 ,监测结核病人体液免疫的可能性。方法 用酶免疫EIA法检测 84例活动结核患者及 35例健康对照血清中抗TBGL抗体。结果 结核患者血清中抗TBGL抗体总阳性率达到 5 5 % ,正常人为 17% (P <0 .0 1)。门诊患者和住院患者的阳性率分别为 36 %和74 % (P <0 .0 0 1)。结论 本法可能用于进行结核的血清学诊断及患者体液免疫功能监测。

Objective To evaluate the method using an enzyme-immunoassay(EIA) in monitoring the humoral immune responses against TB in active TB patients by detection of anti-tuberculous glycolipid(TBGL) antibody.Methods Eighty-four patients with active tuberculosis, 35 healthy controls were subjected in determining the serum IgG anti-TBGL antibody by using an EIA.Results The results showed that the positive rate of anti-TBGL antibody in all active TB patients was as high as 55% while that in normal individuals was 17% (P<0.01). The positive rates of anti-TBGL in outpatients and inpatients were 36% and 74%, respectively (P<0.001).Conclusion The data indicates that this test may be useful for the surveillance of TB prevalence as well as the humoral immune condition of TB patients.