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聚乙烯亚胺介导的报告基因在体外转染效率的研究 被引量：4

Study on the transient transfection efficiency by polyethyleneimine
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摘要 目的　探讨阳离子聚合体载体PEI2 5 (分枝状 ,2 5KDa)在体外的转染效率。方法　PEI2 5和LipofectamineTM2 0 0 0作为转染试剂 ,瞬时转染CHO细胞系 ,通过检测荧光素酶来评估PEI和LipofectamineTM2 0 0 0的转染效率。结果　当N P =5 ,6 ,7时 ,PEI2 5的转染效率与LipofectamineTM2 0 0 0相比无显著性差异 (P >0 .0 5 )。结论　经济实用的PEI适用于体外的瞬时转染。 Objective To investigate the transient transfection efficiency of pGL3-control by PEI25 (polyethylenimine,branched polymer form,25KDa) by in vitro delivery.Methods PEI25 and Lipofectamine TM2000 were used to deliver pGL3-control to CHO cell lines in vitro and the level of luciferase expression was evatualed by detecting the level of expressed luciferase.Results There were no significant difference between N/P=5,6,7 of PEI25 and Lipofectamine TM2000 in the transfection efficiency(P>0.05).Conclusion These results demonstrate that the PEI 25 is efficient for instant delivery of in vitro gene expression.
作者 王宇光 郑建华 赵璐 蔡丽瑛
机构地区 哈尔滨医科大学第一临床医学院妇产科
出处 《哈尔滨医科大学学报》 CAS 2004年第5期426-428,共3页 Journal of Harbin Medical University
基金 黑龙江省十五攻关项目 (GB0 2C13 9 0 2 )
关键词 聚乙烯亚胺 转染效率 荧光素酶 质粒pG13-control Lipofectamine^TM 2000 polyethyleneimine (PEI) transfection efficiency luciferase pGL3-control lipofectamine TM2000
分类号 Q782 [生物学—分子生物学]
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参考文献4

  • 1[1]Ahn CH,Chae SY,Bae YH,et al.Biodegradable poly(ethylenimine)for plasmid DNA delivery[J].J Control Release,2002,80(1-3):273-282.
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同被引文献27

  • 1储明星,何远清,王金玉,Olowofeso Olajide,叶素成,方丽.绵羊肌细胞生成素基因外显子1的PCR-SSCP分析[J].农业生物技术学报,2005,13(1):77-80. 被引量：23
  • 2林磊,熊伟,祝庆余.非病毒型载体介导基因转染[J].医学分子生物学杂志,2007,4(1):36-40. 被引量：4
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哈尔滨医科大学学报
2004年 第5期

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