中国汉族反社会人格障碍人群SLC6A基因启动子区基因多态性的分析

Association Between Human SLC6A4 Gene Promoter Polymorphism and the Chinese People with Antisocial Personality Disorder

目的:探讨中国汉族人群SLC6A4基因启动子区基因多态性与反社会人格障碍的关系。方法:反社会人格障碍人群来自江苏省少年劳教所的被劳教人员,经人格诊断问卷(PDQ鄄4)筛查,符合美国精神病协会精神疾病诊断方法(DSM鄄Ⅳ)关于反社会人格障碍的诊断标准;对照组来自健康体检人群,经PDQ鄄4排除反社会人格障碍和重型精神病。全部对象取血液标本提取DNA,用PCR方法扩增SLC6A4基因(5-羟色氨转移蛋白基因,5鄄HTT)启动子区序列片断,部分PCR产物行测序验证。对等位基因频率和基因型频率进行对比分析。结果:符合DSM鄄Ⅳ反社会人格障碍诊断标准者共90例,对照组140例,PCR产物电泳及测序比对,共获得3种等位基因条带L、S和XL,片断长度各相差44个碱基,分别组成4种基因型LL、LS、SS和XLL。反社会人格障碍人群S等位基因频率和SS基因型频率分别为61.80%和37.08%,与对照组比较,差异有极显著性意义(P<0.005)。结论:S等位基因频率和SS基因型频率与中国汉族人群反社会人格障碍的遗传易感性相关。

Objective: To determine the association between the human SLC6A4 gene promoter polymorphism and the Chinese people with antisocial personality disorder(APD). Methods: Ninety chinese with APD and 140 chinese controls were examined. All subjects were evaluated by PDQ-4,then a diagnosis was made based on the DSM-Ⅳ classifications. The DNA fragments of SLC6A4 promoter region were measured using PCR assay, and verified by sequencing. Both allele frequencies and genotype frequencies in two groups were compared. Results: The PCR products included three allele:L,S and XL, which composed of four genotypes:LL,LS,SS and XLL. The S allele frequence and SS genotype frequence in the APD were 61.8% and 37.08% respectively, which demonstrated a significant difference when compared with controls(P < 0.005). Conclusion: These results suggested an association between the S allele frequence and APD.