菠萝茎蛋白酶的提取及其活性保护研究

THE INVESTIGATION TO PREPARATION OF STEM BROMELAIN AND PROTECTION OF THE ENZYME

本研究结果表明,菠萝茎组织中含有0.54～1.16％的蛋白酶.在酶膏的干燥过程中,45℃烘干使蛋白酶活性下降61.32％,而真空干燥的酶活性只下降18.32％.研究结果发现,硫代硫酸钠和半胱氨酸混用可部分地保护酶活性,使酶活性比对照提高67.37％.在提取中,用氯化钠、醋酸锌、乙二胺四乙酸和抗坏血酸溶液洗涤酶复合物可使酶活性提高110.48％.用0.15～0.25％的丹宁作酶的沉淀剂是适宜的,既可保证酶活性,也可保证有较高的酶产量.

The tissue of pineapple stem contained 0. 54 -1.16% of stem bromelain. It was indicated that the activity of the enzyme desiccated in oven at 45℃ declined by 61.32% and that desiccated by vacuum process declined by 18. 32%. It was discovered that sodium hyposulfite plus cysteine was an effective protector of enzyme activity. It could raise the enzyme activity by 67. 37%. The activity and the quality of the enzyme complex could be improved effectively by cleansing with a solution containing ascorbic acid, disodium edetate. sodium chloride, and zine acetate. The suitable concentration of tannic acid to precipitate stem bromelain was 0. 15-0. 25%.