高效液相色谱法测定血浆或组织中吡喹酮、氯霉素、喹乙醇及利血平的浓度

HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC (HPLC) DETERMINATION OF PRAZIQUANTEL. CHLORAMPHENICOL, OLAQUINDOX AND RESERPINE IN PLASMA OR TISSUES

本研究建立了测定吡喹酮、氯霉素、喹乙醇及利血平浓度的反相高效液相色谱分析方法.以ODS-C18化学键合相(5μm)作为固定相;乙醚萃取血浆中的吡喹酮,组织喹乙醇用乙酸乙酯-乙腈(3:2,V/V)抽提,虾肉氯霉素及组织利血平均用乙酸乙酯提取;甲醇-水为流动相;吡喹酮、氯霉素、喹乙醇及利血平进行紫外检测的波长分别是211、280、260及254nm,最低检出浓度分别是0.02μg/ml血浆、0.02μg/g虾肉、0.2μg/g组织及0.1μg/g组织,方法回收率分别是98.07±3.24、90.12±4.71、71.23±5.49及92.14±5.68％(平均值±标准差).这些方法简单、灵敏、专一,已用于测定猪静注及内服吡喹酮后的血浆药物浓度,对虾饲喂“虾病宝7号”(含氯霉素)后虾肉中氯霉素的残留,鸡内服中毒剂量喹乙醇的组织浓度,以及鸡内服利血平的组织浓度.

The simple and sensitive reversed-phase high performance liquid chromatographic methods were described for the quantitative determination of prazkpiantel. chloramphenicol. olaquinodox and reserpine in plasma or tissues. A reversed-phase ODS-C18 (5μm) column fitted with a UV detector was used for analysis of the four drugs. The wavelengths of detector which was used for the determination of praziquantel. chloramphenicol. olaquindox and reserpine were 211. 280. 260 and 254nm. respectively. Praziquantel in plasma was extracted with ether. Olaquindox in tissues (liver, kidney, muscle, gizzard and fat) was extracted with ethyl acetate-acetonitrile (3 : 2. V/V). Chlorapheni-col in prawn meat and leserpine in tissues (liver. kidney and muscle) were extracted with ethyl acetate. The mobile phases were different ratio of methanol-water. Assay linearity was demonstrated in the range of 0. 02-2. 56 μg prazi-quantel/ml of plasma (r=0.9999). 0. 02- 10μg chloramphenicol/g of prawn meat (r=0.9989). 0.2-6. 4. ugo-laqundox/g of five tissues (r = 0.9961 -0.9987). and 0. 1-6. 4 μg reserpine/g of three tissues (r=0.9975-0. 9992) . The minimum detectable limits of praziquantel. chloramphenicol. olaquindox and reserpine were 4. 0. 4. 0. 10. 0 and 5. 0 ng. respectively. Praziquantel was detectable in 2 ml of plasma to a limit of 0. 02 μg/ml. Chorampheni-col was detectable in 2 g of prawn meat to a limit of 0. 02 μg/g. Olaquindox was detectable in 0. 5 g of each tissue to a limit of 0. 2 μg/g. Reseipint was detectable in 0. 5 g of each tissue to a limit of 0. 1μg/g. The drug recoveries of the extracted procedure of Praziquantel, chloramphenicol. olaquindox and reserpine were 98. 07 ± 3. 24. 90. 12 ± 4. 71. 71. 23± 5. 49 and 92.14±5. 68% (Mean ±SD). respectively. These methods were used to determine the plasma level of praziquantel in pigs after oral and intravenous administration, residues of chloramphenicol in prawn meat after 'Xia-bing-Bao No 7' was orally administered to prawn, the five tissue concentrations of olaquindox in chickens following oral single administration of toxic dosage and multiple oral administration of treatment dosage of the drug, and the three tissue concentrations: of reserpine in chickens after multiple oral administration.