麻竹愈伤组织的诱导培养

INDUCTION AND CULTURE OF CALLUS IN BAMBOO Sinocalamus latiflora (Munro) McClure

麻竹[Sinocalamus latiflora(Munro)McClure(Bamboo)]是一种经济价值高的笋材两用竹。用麻竹笋箨片作外植体在含有2,4—D 4～8mg/L和KT3 mg/L、蔗糖5％、琼脂0.7％～0.8％的MS基本培养基上诱导出愈伤组织.在同样的培养基或添加部分其它养分及激素的培养基上继代培养,愈伤组织能持续生长.

Sinocalamus latiflora (Munro) McClure (Bamboo) has considerable economic importance. Its shoots can be eaten as food and its mature culms used as industrial material. Callus formation from explants of bamboo sprout sheath was induced on Murashige & Skoog basal medium supplemented with the 2, 4-D 4-8 mg/1 and kinetin 3 mg/l and agar 0.7%-0. 8%. Calli could grow continuously when they were subcuitured on the same medium or medium supplemented with other nutrients and hormones.