内源细胞分裂素与菜心花芽分化和菜薹形成的关系

RELATION BETWEEN ENDOGENOUS CTK AND FLOWER BUD DIFFERENTIATION, FLOWERING STALK FORMATION IN Brassica parachitensis Bailey

本试验采用阳离子交换柱、薄板层析和生物鉴定对菜心生育过程内源CTK水平的变化进行了定性鉴定和定量测定。结果表明:菜心的茎端生长点、菜薹及基茎均存在CTK类物质。菜心花芽未分化时茎端生长点的CTK含量逐渐增加,分化前达到小高峰,分化过程逐渐减少。菜薹形成开始时,茎端生长点CTK含量急速回升,抽薹初期(现蕾期)达大高峰,尔后又逐渐降低。菜薹的CTK含量在抽薹初期迅速增加,到抽薹中期达最大,这时正是菜心植株及菜薹鲜重迅速生长时期,抽薹后期逐渐下降。

Changes of endogenous Cytokinins (CTK) during growth and development of Drassica parachinensis were investigated. The results showed that CTK existed in the growing tip, the flowering stalk and the it's base part. The content of CTK in the growing tip increased gradually to a small peak value before flower bud differentiation and then decreased gradually. At the beginning of the flowering stalk formation the CTK content in growing tip rapidly increased ;tgain reaching a big peak value during the early stage, and then decreased. The CTK content in the flowering stalk increased quickly from early to middle stage, and reached the biggest content. During this period, the plant and flowering stalk both grew rapidly.