非放射性探针杂交检测早期钩端螺旋体病患者血清钩体DNA的研究

Detection of Leptospiral DNA in the Serum of Patients with Non-radioactive Labelled Probes

作者首次应用非放射性地高辛配基、光敏生物素探针并以^(32)P探针作对照,斑点杂交检测33创早期钩体病人血清。结果,三种探针杂交的阳性率分别为69.70％(23/33)、27.27％(9/33)、57.58％(19/33)。地高辛配基探针的杂交阳性率高于血培养(20/33),明显优于光敏生物素探针(p<0.01),稍高于^(32)P探针。阴性对照标本中,光敏生物素探针杂交1例出现阳性,地高辛配基,^(32)P探针无阳性结果出现。

DNA of Leptospira interrogans sv.lai strain 017 was lebelled with digoxigenin or bhotobiotin and ^(32)P used as probes to detect DNA of leptospires in the serum of 33 patients. The diagnostic test (proved by 'gold standards' , Clinical diagnosis, blood culture and MAT) showed that positive rate of digoxigenin, photobiotin and ^(32)P probes was 69.70％ (23/33), 27.27％ (9/33)、57.58％ (19/33) of 33 patients, respectively.Therefore, 017 DNA labelled with digoxigenin provided a simple and quick protocol for the diagnostic means of early leptospirosis.