益生菌对烧伤大鼠肠道膜菌群和sIgA的影响

Effects of probiotics on the bacterial groups of intestinal and sIgA in severely burned rats

目的 探索益生菌对严重烧伤大鼠早期肠道菌群的调理作用 ,为防治肠源性感染寻找新途径。方法选用健康Wistar大鼠 10 0只 ,体重 180～ 2 2 0g ,雌雄各半 ,随机分为 :益生菌治疗组 (BR) ,烧伤对照组 (BC)和正常对照组 (NC) ,建立 30 %Ⅲ°烫伤肠源性感染的动物模型 ,按时分批活杀取材 ,检测盲肠膜菌群和肠黏膜sIgA的含量。结果 BC组盲肠中双歧杆菌数量与NC组比较 ,明显减少 (P <0 0 1) ,而BR组与NC组比较 ,差异无显著性 (P >0 0 5 ) ;BC组肠道中酵母菌和大肠埃希菌与NC比较明显增加 ,BR组差异无显著性 ;肠粘液sIgA水平与上述指标有类似变化。结论 30 %Ⅲ°烫伤大鼠肠道内容物中 ,双歧杆菌明显下降 ,大肠埃希菌、酵母菌迅速过生长 ,导致肠道微生态失衡 ,应用BR治疗后 ,促进肠黏膜机械屏障功能的恢复 ,调理肠道微生态平衡 ,对肠黏膜起占位性保护作用 。

Objective To probe the effect of probiotics on preventing translocation of bacteria and endotoxin from the gut.Methods A modle of 30% Ⅲ° TBSA scald rats of 100 were divided randly into normal control(NC),burn control(BC),treatment of probiotics. Biopsies were made to observe of cecum mucosal microflora,changes of sIgA in intestinal mucas.Results Compared that group NC the quantities of Bifidobacteria were not fifferent in probiotics,while in groups BC were reduced (P<0.01).Quantities of yeast and E.coli in group BC were significantly increased.The levels of sIgA in group probiotics were high at any term,while in group BC were significantly low in all term.Conclusions After severe burns,microbes and microbial products,including endotoxin,might across the insulted gut mucosal barrier to cause systemic infection.Bifidobacteria significantly low,E.coli and Yeast were increased,probiotics were useful in improving the situation,however probiotics functions more early and more validly.