摘要
利用酪蛋白培养基和脱脂乳培养基 ,从健康牙鲆肠道筛选出 2 5株产蛋白酶的益生菌 ,占健康牙鲆肠道菌群的 17 7% ,并利用福林 酚试剂法测定其酶活力。结果表明 ,2 5株菌具有产蛋白酶酶活力 ,其中E14、F1、G7和Y2 2株产酶活力特别强 。
Based on the microecological principles,some bacterial stains were isolated and screened from the autochthonous flora of flounders intestine with the common medium and selective medium for the screening of proteinase-producing bacteria. 25 strains were screened to produce proteinase by casein enzyme-produced assay and skimmed milk enzyme-produced assay.In their further study,proteinase activity of strains after cultured 24 h in 25 ℃ were determined by Folin-phenol method.It was indicated that 25 strains had proteinase activity,in particularly E14,F1,G7 and Y2-2.It was demonstrated that some of these strains after further study would be used in flounder as ecological preparation with study on its biological characterization and dereaseing death rate.
出处
《中国微生态学杂志》
CAS
CSCD
2004年第5期259-262,共4页
Chinese Journal of Microecology
基金
辽宁省自然科学资金资助项目 ( 2 0 0 10 2 0 67)