神曲1号、2号对脾虚小白鼠肠道菌群调整及肠保护作用的研究

Reseach regulation of SHENQU Ⅰ and SHENQU Ⅱ to alteration of intestinal flora mice and the intestines protect function

目的 研究神曲 1号、2号对脾虚小白鼠肠道菌群的调整及肠保护作用。方法 (1)昆明小白鼠 ,分成 5组 ,A组 (正常对照组 ) ,B组 (脾虚自然恢复组 ) ,C组 (神曲治疗组 ) ,D组 (神曲 1号治疗组 ) ,E组 (神曲 2号治疗组 )。 (2 )用神曲中筛选出的酵母菌、几种中药和其他配料制成液体神曲 1号、2号 ,用于实验。 (3)检测结肠内容物和粪便标本的肠杆菌、肠球菌、双歧杆菌、类杆菌和乳酸杆菌数量。 (4 )结肠组织光镜和电镜观察与一般状态观察。结果 脾虚小白鼠给予神曲、神曲 1号、2号治疗后 ,肠杆菌、肠球菌、双歧杆菌、类杆菌和乳酸杆菌数量逐渐恢复正常 ,肠壁肌层厚度增加 ,杯状细胞数量增多 ,肠黏膜微绒毛排列紊乱、线粒体肿胀显著改善 ,明显好于脾虚自然恢复组。D组、E组一般状态改善优于A、B和C组。结论 神曲 1号、2号对脾虚小白鼠具有调整肠道菌群及促进损伤肠组织恢复的作用 。

Objective To study the regulation of Shenqu Ⅰand Shenqu Ⅱ to alteration of intentional flora spleen deficiency mice and the intestines protect function .Methods KUN MING white mice were divided into five groups:normal contrast group,Shenqu Ⅰ group,Shenqu Ⅱ group,natural cure group.Shenqu Ⅰand Shenqu Ⅱ solution were used in the experiment,being made up of yeasts from Shenqu,several traditional Chinese drugs and other batching.The quantities of main bacteria in colonic contents and faeces of mice were determinate such as Enter obacteriaceae,Intestines coccue,Bifidobacterium,Bacterocdaceae and Lactobacillus.Observed colon organize with potical microscope and electeron microscope.Results After one course of treatment with Shenqu Ⅰ and Shenqu Ⅱ,the quantities of Enterobacteriaceae,Intestines coccus,Bifidobacterium,Bacterocdaceae and Lactobacillus returned to normal gradually,intestine wall skin layer of thickness increase,cup form cell quantity increase,intestines mucous membrame little fine grrange order,mitochondria swelling improve notably.Obviously better than natural cure group,Shenqu Ⅰand Shenqu Ⅱ group hadn't eremarkable difference from Shenqu group.Conclusion Shenqu Ⅰ and Shenqu Ⅱ had an affect to regulate dysbateriorsis and promote recovery of colon damaged.