高效液相色谱法测定发酵液中有机酸

Determination of Organic Acids in Fermentation Liquor by High Performance Liquid Chromatography

建立反相HPLC测定酒石酸、苹果酸、乳酸、醋酸柠檬酸的色谱条件,在ODS柱上以0.3mol/LKH_2PO_4(pH2.5)缓冲溶液作流动相,10min内完成以上五种有机酸的同时测定。检测限低于10ng/ml,线性范围0—800μg/ml。

For determining tartaric acid, malic acid, lactic acid, acetie acid and citric acid in fermentation liquor by reversed phase high performance liquid chromatography (HPLC), 0.3 mol/L KH_2PO_4 buffer (pH 2.5)is taken as mobile phase and the conditions of chromatogra- phy are established. The above five organic acids are determined simultaneously on ODS col- umn in 10 minutes, with a limit of detection below 10ng/ml and a linear range of 0-800 μg/ ml.