摘要
通过3种动物、2种自由基发生体系、不同药物浓度与2种室温的偶联比较,得出建立研究氧自由基损伤口腔粘膜的动物模型的条件是:选用叙利亚金黄地鼠双侧颊囊粘膜下注射甲基紫精0.25ml,20~22℃饲养观察24小时以上;电子顺磁共振证实该模型确由氧自由基所致。
Three kinds of animal(white rat,mouse and hamster),two free radical generation systems(Xanthine-Xanthine oxidase system and paraquat system)had been coupled with 2 differentroom temperatures(15℃ and 20—22℃).After being compared,the best condition that could beused to research the damage of oxygen (?)r(?)e radicals to the oral mucosa of animal had been gotten,It was that 0.25ml paraquat was injected into the cheek pouches of Syrian hamster bilaterally,fedat 20—22℃ and observed more than 24 hours.The signals of oxygen free radicals had been de-tected by electron spin resonance(ESR).So the authors believe that the animal model used tostudy the damage of oxygen free radicals to the oral mueosa is established for the first time.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1993年第1期62-64,共3页
West China Journal of Stomatology
基金
国家教委基金资助课题:编号C19005