组织形态计量相关分析法在颊粘膜癌前病变诊断中的意义

Morphometric Correlative Analysis(MCA)and Its Potential Applicationto Diagnosis of Premalignant Lesions of the Human Cheek Epithelium

用形态计量学方法对口腔颊粘膜的正常粘膜上皮(N)、慢性非特异性炎症(IF)、单纯性过角化(HK)、纤维增生(FH)、扁平苔藓(LI)、白斑(LP)、乳头状瘤(PP)、癌旁上皮(CE)、鳞癌癌巢(CI)的上皮基底层细胞及胞核的大小(包括体积、面积和胞核/胞浆比值)、进行了测量和形态计量相关分析(MCA)。结果显示 CI 组在分布图上最偏离 N 组,其次是 CE 组,LI 与 LP 组位置非常接近并邻近于 CE和 CI 组;此外发现 IF,FH,HK 及 PP 组与 N 组的位置最为接近。对于病变个体的 MCA 分析显示 N 组最为集中,IF,FH,HK 及 PP 的分布相对比较集中,围绕或与 N 组相重叠;而 CI 组与 N 组无重叠且偏离一边,LI,LP 及 CE 组位于 CI 与 N 及其它良性病变组之间并部分与 CI 重叠。本文结果揭示通过 MCA方法可以较好的反映颊部粘膜各病变所处的病变状态、各病变之间的关系以及个体病变的病变程度。应用 MCA 的方法将有助于癌前病变的早期发现以及确定癌变组内的不同级别。

In present report,a morphometric method with VIDAS system was firstly adopted for estimating the histologi-cal changes of the dimensions(Volume,area)and N/C ratio of basal cells of cheek epithelium.Following histologi-cal groups were observed:normal mucosa(N),unspecific inflammation(IF),fibrous hyperplasia(FH),hyperkerato-sis(HK),lichen planus(LI),leukoplakia(LP),squamous cell papilloma(PP),dysplastic epithelium immediately ad-jacent to squamous cell carcinoma(CE)and the islands of carcinomatous epithelium(CI).Then the correlative analy-sis was done with the results of the above measurement.The findings showed that the correlation between N/C ra-tio and the dimensions of cell and nucleus,and their scattering plot could indicate the relationship between normaland lesions and within different lesions.In the present study,the distribution of CI was the fartherst one divergingfrom the normal group on the scattering plot,and the second was CE whick closely near to CI.The distributing po-sition of LI adjacented to LP,both of them near to CE and CI.The observation of the scattering of the individualdata showed that,part of the individual data of LP,LI and CE over lapped CI,and also another small part of themcovered the other benign lesions.The most concentrating distribution was found in normal,and then IF,FH andHK.The individual data of IF,FH and HK were scattered around the normal group.The site of PP on the plot wasnear to the benign lesions.The results indicated that the further study using this method can be beneficial to ana-lyze,diagnose and prognose the high risk premalignant lesions.