反相高效液相色谱法测定和比较厚朴及和厚朴中厚朴酚及和厚朴酚含量

DETERMINATION AND COMPARISON OF MAGNOLOL AND HONOKIOL IN MAGNOLIA OFFICINALIS AND MAGNOLIA OBOVATA BY RP-HPLC

采用反相高效液相色谱法对厚朴与和厚朴生药进行含量测定与比较,结果表明:供试和厚朴生药中厚朴酚与和厚朴酚含量均高于厚朴生药,该分析测定方法简便,灵敏,准确。

Magnolol and Honokiolwere separated on a ODS column, 150×4mm id(Speriso b C18 5μm), using met-hanol-0.5% phospheric acid (70:30) as themobile phase. The flow rate was 1.0 ml/min and the detection wavelength was294 nm. The detection limit of Magnololand Hnolokiol were 5.7 ng, 3.2 ng, res-pectively,which was determined by thepeek area measurement using an externalstandard method. The calibration curvesof Magnolol was linear in the range of16-25000 ng(r=0.9998), and Honokiol waslinear in the range of 8.32-13000 ng(r=0.999). The average recoverys of Maguololwas 100±1.37% (n=9), and Honokiol was99.91±1.37% (n=9). Two conventional e-xtracting mothods were applied to extractMagnolol and Honokiol, which showedno significant difference. Both Magnololand honokiol were higher in Magnoliaobovata than in Magnolia officinalis. The estabilished determination mothodwas successful1y applied to crude Magn-olis from different origins.