化学发光自显影检测人血中巨细胞病毒抗体

CHEMILUMINESCENT PHOTOGRAPHIC DETECTION OF HUMAN CYTOMEGALOVIRUS-Ab

本文报道用化学发光自显影技术(Chemiluminescent photographic detection,CPD)与点免疫测定(Dot-immunobinding assay,DIA)结合检测了人血中巨细胞病毒(HCMV)抗体。从氧化剂、发光增强剂、缓冲液种类优选及浓度优选找出了化学发光废物液的最佳配方。以硝酸纤维素膜(NC)为载体,用x-光胶片记录化学发光,检测辣根过氧化物酶(HRP),检测限2×10^(-15)～10^(17)mol。用NC以来心法检测HCMV-Ab,与常规ELISA法对比,57例样品中,定性判断阴阳性彼此符合48例,符合率为84.2%(48/57),但CPD法所用酶标抗体浓度较ELISA法小千倍。

In this paper is reportedchemiluminescent photopaphic detect-ion(CPD) joint with dot-immunobindingassay(DIA) to detect antibody againsthuman cytomegalovirus (HCMV) in se-rum. The oxidant, chemiluminescenceenhancer, their amount and the bufferwere optimized.Nitrocellulose(NC) filterwas used as the solid support for dete-cting pure horseradish peroxidase(HRP)and HCMV-Ab, and X-ray film used torecord the ligh emission. The detectionlimit of HRP was in the range of 2x10^(-18) ～10^(17) mol. The results of CPD for57 samples were compared with those ofconventional ELISA, the rate of agree-ment of the two methods being 84.2%(48/57). Mean-while, the concentrationof HRP labeled antibody used in CPDwas only 1/1000 of that in ELISA.