女贞子血清药理对Hela细胞增殖抑制的实验研究

Serum pharmacological effect of FL on Hela cell

目的 :观察女贞子血清药理对宫颈癌 Hela细胞增殖抑制作用。方法 :应用血清药理学方法研究中药的体外效应 ,用四甲基偶氮唑蓝 (MTT)比色法检测含女贞子兔血清对 Hela细胞的抑制效应 ,并采用末端标记法 (TU NEL )对凋亡细胞进行定量检测。结果 :中药兔血清对 Hela检测结果为女贞子血清促进 Hela细胞凋亡。结论 :女贞子血清药理抑制 Hela细胞增殖 。

Objective:To observe whether the serum pharmacological effect of FL inhibits Hela cell proliferation in cervical cancer.Methods:We used MTT assay to examine the serum pharmacological effect of FL on the decrease im Hela cell,we used TUNEL for quantitive determination of the apoptosis of Hela cell.Results:The FL serum accelerated Hela cell apoptosis..Conclusion:The serum pharmacological effect of FL controls proliferation of Hela cell.which is very important for the gaidance in clinical treatment by the drug.