血清药理学方法研究蛇葡萄素抗B16黑色素瘤的作用

Study on Inhibitory Effect of Ampelopsin on Melanoma by Serologic Pharmacological Method

目的:研究蛇葡萄素对黑色素瘤的抑制作用。方法:以血清药理学方法考察不同剂量的蛇葡萄素含药血清对B16黑色素瘤细胞的抑制作用,分别用MTT法及流式细胞术进行评价。结果:0.420mmol/kg、0.560mmol/kg剂量组的C57BL/6小鼠含药血清能有效抑制黑色素瘤B16细胞,与空白血清中加入浓度相当的蛇葡萄素的抑制率相同;两个剂量组的含药血清使肿瘤细胞的分裂增殖指数分别降低了19.1％、21.7％。结论:蛇葡萄素在血药浓度达峰期对小鼠黑色素瘤B16细胞的抑制可能以原型物形式起作用,抑制细胞的DNA合成。

Objective:To study the inhibitory effect of ampelopsin on melanoma. Methods: Using a serologic pharmacological method, the inhibitory effects of different dose of ampelopsin on B16 melanoma cells were evaluated by a MTT assay and a flow cytometry. Results: The serums of C57BL/6 mices, which were administered by 0.420 mmol/kg dose and 0.560 mmol/kg dose of ampelopsin, could obviously suppress the B16 melanoma cells, and the inhibitory rate was equal to that of the vacant serum adding same consistency ampelopsin. The drug serums of the two doses groups reduced the cleavage and proliferation index of B16 melanoma cells to 19.1% and 21.7% respectively. Conclusion: Ampelopsin possibly acts on melanoma B16 cells by original form drug at the period with a peak of the drug concentration, and inhibits the DNA synthesis of B16 cells.