茵栀黄颗粒的保肝作用研究

Study on Hepatic-protective Effect of "Yinzhihuang" Granula

目的:研究茵栀黄颗粒的保肝作用。方法:采用D-氨基半乳糖(D-GalN)、四氯化碳(CCl_4)造成急性肝损伤,以血清ALT与AST、肝组织病变程序为指标,并以茵栀黄颗粒口服液为阳性对照,观察茵栀黄颗粒的药效。另外,给小鼠ig异硫氰酸-1-奈脂,以血清胆红素为指标,观察茵栀黄颗粒的作用。结果:1.8g/kg、3.6g/kg茵栀黄颗粒组均能降低D-GalN所致的小鼠血清ALT、AST升高,3.6g/kg茵栀黄颗粒组还能减轻肝组织病变程度。1.0g/kg、2.0g/kg茵栀黄颗粒组均能降低CCl_4所致的大鼠血清ALT、AST升高,2.0g/kg茵栀黄颗粒组还能减轻肝组织病变程度。1.8g/kg、3.6g/kg茵栀黄颗粒组均能降低异硫氰酸-1-奈脂所致总胆红素和结合胆红素升高。结论:茵栀黄颗粒对D-GalN、CCl_4所致的急性肝损伤均有明显的保护作用,对异硫氰酸-1-奈脂所致小鼠血清高胆红素有明显的降低作用。

Objective:To study hepatic-protective effect of 'Yinzhihuang' granula.Methods:By D-GalN,CCl4 induced acute hepatic damage model, ALT and AST in serum,pathological degree of hepar adopted as indexes, the potency of Yinzhihuang granula was observed and compared with 'Yinzhihuang oral solutions.Mice administrated ig 1-naphthyl-isothiocyanate,with bilirubin in serum used as index,the effect of Yinzhihuang granula was observed. Results:1.8 g/kg,3.6 g/kg Yinzhihuang granula groups could decrease the rising of ALT, AST in mice serum induced by D-GalN.3.6 g/kg 'Yinzhihuang' granula group could also improve pathological degree of hepar. 1.0 g/kg, 2.0 g/kg 'Yinzhihuang' granula groups could decrease the rising of ALT, AST in rats serum induced by CCl4.2.0 g/kg 'Yinzhihuang' granula group could also improve pathological degree of hepar. 1.8 g/kg,3.6 g/kg 'Yinzhihuang' granula groups could decrease the rising of bilirubin in mice serum induced by 1-naphthyl-isothiocyanate.Conclusions:'Yinzhihuang granula had an evident hepatic-protective effect on acute hepatic damage induced by D-GalN or CCL4,and could decrease the rising of bilirubin in mice serum induced by 1-naphthyl-isothiocyanate.