广藿香组织培养与植株再生研究

Study on the Tissuse Culture and Plant Regeneration of Pogostemon cablin

以广藿香Pogostemon cablin(Blanco)Benth的根尖、叶片、带节茎段及茎段为材料进行培养,结果表明:叶片及带节茎段较易诱导愈伤组织,诱导率均为87.0％以上,适宜的培养基为MT+BA 0.5mg/L+NAA 0.2mg/L。愈伤组织的分化与增殖,以MT+BA 0.5～1.0mg/L为好。在MT基本培养基中,组培苗就能形成根。

Root tips,leaf segments,stem segments with node and stem segments of Pogostemon cablin (Blanco) Berth, were cultured in vitro.The results indicated that leaf segments and stem segments with node were suitable for callus formation, the frequency of callus induction were over 87.0%.A optimal medium for callus induction was MT+ BA 0.5 mg/L+ NAA 0.2 mg/L.MT basal medium with BA 0.5-1.0 mg/L were effective to induce differentiation of callus. And at the MT basal medium, the regenerative shoots from Pogostemon cablin could form roots.