三七及其伪品的DNA测序鉴别

Identification of Notoginseng(Panax notoginseng) and Its Adulterants Using DNA Sequencing

目的:分析三七Panax notoginseng及其伪品竹节参P.japonicus、蓬莪术Curcuma phaeocaulis、温莪术C.wenyujin、桂莪术C.kwangsiensis的核基因和叶绿体基因序列,为三七的正品药材基原鉴定提供分子依据。方法:采用PCR直接测序技术测定三七及其4种伪品的18S rRNA基因和matK基因核苷酸序列并作序列变异分析。结果:三七和竹节参的18S rRNA基因序列长度相同,均为1809 bp;matK基因长度亦相同,为1259 bp。蓬莪术、温莪术和桂莪术18S rRNA基因长度均为1811 bp、matK均为1548bp。根据排序比较,三七与4种伪品间的DNA序列存在很大差别。结论:通过序列差异比较分析,DNA测序技术可成为三七正品基原鉴定的准确、有效手段。

Objective: To analyze the nuclear ribososmal RNA small subunit (18S rRNA) and chloroplast matK gene sequence of notogin-seng ( Panax notoginseng) in order to provide molecular evidence for its genuine origin identification. Methods: To sequence 18S rRNA and matK genes of Panax notoginseng and its four adulterants such as P. japonicus , Curcuma phaeocaulis , C. wenyujin, C. kwangsiensis using PCR direct sequencing and to detect their variation of sequences. Results: The sequence length of notoginseng and its adulterants is 1809-1811 bp for 18S rRNA gene and 1259-1548 bp for matK gene, respectively. Multiple sequence alignment shows that there are much sequence variation between notoginseng and its adulterants. Conclusion: DNA sequencing is an accurate and reliable method in origin identification of the genuine notoginseng.