噻唑蓝法检测5种抗癌药物对人癌细胞的体外抑制作用

Studies on Inhibiting Activities of Five Antitumour Drugs to Human Cancer Cell in vitro with MTT Assay

用噻唑蓝比色法测定安克素等5种抗癌药物对19例人癌细胞的体外抑制率,为临床肿瘤化疗提供用药选择。药敏的可评价率为(17/19)89.47％,资料与临床基本相符。该测试能够反应肿瘤群体细胞与安克素等药物的关系,方法简单、灵敏、快速,具有实用价值及临床意义。

The inhibiting activities of five antitumour drugs including 'An ke su' to human cancer cell lines coming from 19 patients were measured in vitro with MTT assay, which can provide proper antitumor agents for ch'nical chemotherapy.The evaluation rate of medicine sensitivity is 89.47% , which is same as the result of clinical therapy .This method indicates the response of tumour cells to An Ke Su and other a-gents.Being simple,sensitive and rapid,it proves to be valuable in clinical therapy.