非特异性T细胞疫苗对空肠弯曲菌诱导的SLE样综合征小鼠免疫功能的影响

The effects of non-specific T cell vaccination on immune function of systemic lupus erythematosus-like mouse model induced by campylobacter jejuni

目的探讨非特异性T细胞疫苗(TCV)对空肠弯曲菌诱导的系统性红斑狼疮(SLE)样综合征小鼠免疫功能的影响。方法采用甲醛化空肠弯曲菌CF-1株辅以佐剂诱导近交系BALB/c小鼠SLE样自身免疫综合征病理模型,然后用非特异性TCV对该病理模型进行治疗。用酶联免疫吸附试验(ELISA)法检测小鼠血清抗dsDNA抗体、抗空肠弯曲菌CF-1外膜蛋白(CF-OMP)抗体水平,并检测特异性迟发型超敏反应(DTH),用苏木素-伊红(HE)染色检测肾脏病损。结果①非特异性TCV一方面使模型鼠抗dsDNA抗体水平升高,另一方面却使血清抗CF-OMP抗体水平一定程度的降低;②非特异性TCV明显抑制CF-1模型鼠对空肠弯曲菌特异性的DTH反应;③非特异性TCV能部分减轻模型鼠的肾脏病损。结论非特异性TCV对模型鼠的体液免疫和细胞免疫均有抑制作用。

Objectives To study the effects and pathogeneic mechanism of non-specific T cell vaccination (TCV) on immune function of systemic lupus erythematosus (SLE)-like mouse model induced by campylobacter jejuni. Methods SLE-like mouse model was established by immunizing syngeneic mouse BALB/C with campylobacter jejuni strain CF-1, and then treated with non-specific TCV. Results The chara-cteristics of mouse model induced by CF-1 were high level serum autoantibodies against dsDNA and CF-OMP, enhanced DTH reaction and polyorganic inflammatory injury. The immunized mice can be treated by non-specific TCV. The results demonstrated that serum specific anti-CF-OMP antibodies were decreased and DTH reaction was suppressed and kidney injury was partially recovered. Conclusions Non-specific TCV can suppress both humoral and cellular immune function.