斜纹夜蛾NPV在半受纳细胞中复制的初步研究

PRELIMINARY STUDY OF REPLICATION OF SPODOPTER.A LITURA NPV IN INSECT SEMIPERMISSIVE CELL LINE

斜纹夜蛾NPV感染半受纳细胞——莲纹夜蛾细胞后,细胞产生一定程度的病理效应,受染细胞表面粗糙,细胞核肿胀膨大。超薄切片电镜观察显示受染细胞核中形成电子致密的病毒发生基质与大量分布在病毒发生基质中间及周围的核衣壳,但并没观察到具囊膜的、成熟的病毒粒子和多角体。莲纹夜蛾细胞不能提供斜纹夜蛾NPV完全复制,病毒在半受纳细胞中的复制与增殖受到限制,限制可能发生在病毒粒子装配阶段。而斜纹夜蛾NPV能在其同源细胞中正常复制。

Replication of spodoptera litura nuclear polyhedrosis virus in spodoptera littoralis cell line (Semipermissive cell) was studied. After infected by slNPV cells produced limited cytothic effect. Surface of infected cells was rough and infected cells exhibited hypertrophy. 120hr postinfection electron dense virogenic stroma and nucleocapsides were observed in nucleus of infected cells by electron microscope. Neither enveloped virus particles nor PIBS were observed at any time during the slNPV infection of S. Li cells. S. Li cells could not support slNPV comlet replication. Replication of slNPV in S. Li cell was restricted. The restriction perhaps occured at virion assambly stage, slNPV could normaly replicate in its homologous spodoptera litura cell line (sL-1).