摘要
目的 :寻找中药康莱特抗癌症恶病质的表达差异基因。方法 :复制 T739小鼠肺腺癌恶病质模型 ,制备 m RNA探针 ,应用 c DNA表达谱芯片 ,分析差异表达的基因。结果 :得到的康莱特治疗组差异表达基因 5条 ,其中 1条表达降低 ,4条表达增高 ,多数差异表达基因与代谢、免疫相关。结论 :运用基因芯片工具和生物信息学方法 ,能够发现康莱特治疗癌症恶病质相关的差异表达基因 。
Objective: To screen the differential expression genes of Kanglaite Injection in treating cancer cachexia. Methods: mRNA was extracted from the blood cells of T739 animal model of C.C., hybridizated respectively on 20S gene chip. Analysis discuss on differential expression genes was carried out. Results: 5 differential expression genes were obtained. Among these genes, 4 genes were up-regulated and 1 gene was down-regulated. Most of these genes were related with immunity and metabolism of tumor. Conclusion: cDNA microarray for analysis of gene expression patterns is a powerful method to identify associated genes of Kanglaite.
出处
《浙江中西医结合杂志》
2004年第11期664-666,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
20 0 1年度国家中医药管理局课题:2 0 0 0 -J-B-2 5
